Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells
نویسندگان
چکیده
منابع مشابه
Use of automated microscopy for the detection of disseminated tumor cells in bone marrow samples.
The use of automated microscopy has reached the maturity necessary for its routine use in the clinical pathology laboratory. In the following study we compared the performance of an automated microscope system (MDS) with manual method for the detection and analysis of disseminated tumor cells present in bone marrow preparations from breast carcinoma patients. The MDS System detected rare dissem...
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BACKGROUND Approximately half of the patients diagnosed with localized esophageal cancer die of metastatic disease within the first 2 years following tumor resection. The development of monoclonal antibodies (MAbs) directed against epithelial cell-associated and tumor antigens has enabled the detection of single disseminated tumor cells in secondary organs. PURPOSE We used MAbs directed again...
متن کاملBone marrow carcinosis and disseminated tumour cells.
to be deemed bone marrow carcinosis. Matrix bone metastases were demonstrated in 78% of patients with bone marrow carcinosis versus only 16% of the patients without bone marrow carcinosis. Heavy tumour infiltration of the bone marrow was associated with multiple bone lesions with histopathologic evidence of bone destruction [5]. This study provided evidence that the primary soil of metastatic b...
متن کاملEnrichment and Molecular Analysis of Breast Cancer Disseminated Tumor Cells from Bone Marrow Using Microfiltration
PURPOSE Molecular characterization of disseminated tumor cells (DTCs) in the bone marrow (BM) of breast cancer (BC) patients has been hindered by their rarity. To enrich for these cells using an antigen-independent methodology, we have evaluated a size-based microfiltration device in combination with several downstream biomarker assays. METHODS BM aspirates were collected from healthy volunte...
متن کاملHer2 expression on disseminated tumor cells from bone marrow of breast cancer patients.
BACKGROUND The presence of cytokeratin (CK)-positive cells in the bone marrow (BM) of breast cancer patients is an independent prognostic marker for long-term survival. While the exact nature of these cells remains under investigation, their persistence after chemotherapy is linked to decreased survival, making them a potential therapeutic target. Targeted therapy using the her2 antibody has be...
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ژورنال
عنوان ژورنال: Biological Procedures Online
سال: 2018
ISSN: 1480-9222,1480-9222
DOI: 10.1186/s12575-018-0078-5